Daniela Ross-Kaschitza

Research Scientist

I joined Abriel’s group in January 2018 and I am currently working as a scientific research assistant on the investigation of ion channel

expression and regulation, as well as channel-channel interactions.

My projects mainly include protein biochemical, molecular biological, but also cell biological or analytical methods, such as a) transcript and protein expression studies (RT-qPCR, Western Blot, biotinylation assay) of variants expressed in HEK293 or CHO cells or of endogenous proteins from isolated murine cardiomyocytes; b) protein-protein interaction studies (Co-IP, Proximity Ligation Assays); c) investigations of tagged protein expression and purification for subsequent CryoEM studies, in collaboration with the EPFL Lausanne and University of Basel; and d) analysis of novel small-molecule modulators on ion channel total or surface expression, in collaboration with the Department of Chemistry, Bern.

Prior, during and after my PhD graduation in the group of Prof. Michael Altmann (IBMM, University of Bern), I was interested in deciphering the molecular mechanisms of translation initiation from mRNAs to proteins.

In my spare time I try to spend as much time as possible outdoors: strolling through the woods, hiking or gardening, always with the camera in my hands to capture any beautiful moments.

Publications

S. Casini et al., “Functional Consequences of the SCN5A-p.Y1977N Mutation within the PY Ubiquitylation Motif: Discrepancy between HEK293 Cells and Transgenic Mice,” International Journal of Molecular Sciences , vol. 20, no. 20. 2019.

S. Kappel et al., “TRPM4 is highly expressed in human colorectal tumor buds and contributes to proliferation, cell cycle, and invasion of colorectal cancer cells,” Mol. Oncol., vol. 13, no. 11, pp. 2393–2405, Nov. 2019.

L. C. Ozhathil et al., “Identification of potent and selective small molecule inhibitors of the cation channel TRPM4,” Br. J. Pharmacol., vol. 175, no. 12, pp. 2504–2519, Jun. 2018.

N. Arndt, D. Ross-Kaschitza, A. Kojukhov, A. A. Komar, and M. Altmann, “Properties of the ternary complex formed by yeast eIF4E, p20 and mRNA,” Sci. Rep., vol. 8, no. 1, p. 6707, 2018.

D. Ross and M. Altmann, “eIF4Es and Their Interactors from Yeast Species,” Hernández G., Jagus R. Evol. Protein Synth. Mach. Its Regul. Springer, Cham, 2016.

D. Ross and M. Altmann, “A fast protocol for purification of translating mRNAs,” Methods, vol. 107, pp. 57–62, 2016.

D. Ross, M. Saxena, and M. Altmann, “eIF4E Is an Important Determinant of Adhesion and Pseudohyphal Growth of the Yeast S. cerevisiae,” PLoS One, vol. 7, no. 11, pp. 1–8, 2012.

On the Web

Email: daniela.ross@ibmm.unibe.ch

Institute of Biochemistry and Molecular Medicine

(ORCID 0000-0003-1367-0671)