I am investigating the role of ion channels linked to heart disorders. I mainly focus on one ion channel, the calcium channel Cav1.2.
During my scientific carrier started in 2002, I have investigated different aspects of several important cardiac ion channels involved in the generation of the cardiac action potential, with a strong focus on Nav1.5 and Cav1.2. We have shown the Nedd4-dependent negative regulation of Nav1.5 and Cav1.2 channels. In addition, we also demonstrated that the counterpart of Nedd4-1, USP2-45, regulated Cav1.2 channel. We have highlighted the regulation of Nav1.5 channel via the syntrophin/dystrophin/utrophin complex in mouse ventricular cardiomyocytes. We reported also the regulation of cardiac Ca2+ and Na+ currents by mineralo- and glucocorticoids and the implication of the mutated auxiliary subunit of Cav channel, Cavα2-δ1, in a new type of cardiac pathology: The short QT syndrome type 6 (SQT6). Finally, we reported the role in vivo of different members of the Membrane-Associated GUanylate Kinase (MAGUK) family: the Synapse-Associated Protein-97 (SAP-97) and the Calcium/calmodulin-dependent Serine protein Kinase (CASK) into the regulation of the voltage gated sodium channel Nav1.5.
For the future, I plan on investigating voltage-gated calcium channel and the role of its auxiliary subunits.
On the Web
Amarouch MY, Zaklyazminskaya EV, Rougier JS. Editorial: Inherited Arrhythmias of the Cardiac Sodium Channel Nav1.5. Front Physiol. 2021. doi: 10.3389/fphys.2021.716553.
Ozhathil LC, Rougier JS, Arullampalam P, Essers MC, Ross-Kaschitza D, Abriel H. Deletion of Trpm4 Alters the Function of the Nav1.5 Channel in Murine Cardiac Myocytes. Int J Mol Sci. 2021. doi: 10.3390/ijms22073401.
Vermij SH, Rougier JS, Agulló-Pascual E, Rothenberg E, Delmar M, Abriel H. Single-Molecule Localization of the Cardiac Voltage-Gated Sodium Channel Reveals Different Modes of Reorganization at Cardiomyocyte Membrane Domains. Circ Arrhythm Electrophysiol. 2020. doi: 10.1161/CIRCEP.119.008241.
Gillet L, Guichard S, Essers MC, Rougier JS, Abriel H. Dystrophin and calcium current are decreased in cardiomyocytes expressing Cre enzyme driven by αMHC but not TNT promoter. Sci Rep. 2019. doi: 10.1038/s41598-019-55950-w.
Rougier JS, Essers MC, Gillet L, Guichard S, Sonntag S, Shmerling D, Abriel H. A Distinct Pool of Nav1.5 Channels at the Lateral Membrane of Murine Ventricular Cardiomyocytes. Front Physiol. 2019. doi: 10.3389/fphys.2019.00834.